Language

Package r-hsmmsinglecell @ 1.2.0

Synopsis

Single-cell RNA-Seq for differentiating human skeletal muscle myoblasts (HSMM)

Description

Skeletal myoblasts undergo a well-characterized sequence of morphological and transcriptional changes during differentiation. In this experiment, primary human skeletal muscle myoblasts (HSMM) were expanded under high mitogen conditions (GM) and then differentiated by switching to low-mitogen media (DM). RNA-Seq libraries were sequenced from each of several hundred cells taken over a time-course of serum-induced differentiation. Between 49 and 77 cells were captured at each of four time points (0, 24, 48, 72 hours) following serum switch using the Fluidigm C1 microfluidic system. RNA from each cell was isolated and used to construct mRNA-Seq libraries, which were then sequenced to a depth of ~4 million reads per library, resulting in a complete gene expression profile for each cell.

Home page
https://www.bioconductor.org/packages/HSMMSingleCell/
Location
gnu/packages/bioconductor.scm (line: 997, column: 2)
License

Lint warnings

LinterMessageLocation
formatting

Look for formatting issues in the source

line 1010 is way too long (95 characters)